Talk:20.109(S09):Induce protein and evaluate DNA (Day5)

Examples of induced (left) and control (right) cell pellets

TR O/N cultures and Gels

The table below lists the OD₆₀₀ values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group	OD WT	OD S101L	OD Chosen mutant	Pellets yellow?
Pink	0.117	0.098	0.126	Yes
Yellow	No finished samples	N/A	N/A	No
Red	0.121	0.187	0.133	Yes

Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V.

T/R Gel, Yellow group

T/R Gel, Orange group

T/R Gel, Blue group

T/R Gel, Grey group

T/R Gel, Red group

T/R Gel, Pink group

Teaching example

T/R Gel, Green group

T/R Gel, Purple group

There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which is posted above. Unfortunately, some of the bands are faint. Lane 1 is parent IPC (only one form of the plasmid is visible), lane 2 is the ladder, lane 4 is the parent digested with FspI, and lane 8 is the S101L mutant digested with FspI. The short band is very faint, but it is present. I will have the original photo available for viewing for anyone who wants to clearly see the short band.

WF O/N cultures and Gels

The table below lists the OD₆₀₀ values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group	OD WT	OD Mut1	OD Mut2	Pellets yellow?

pics here