Talk:20.109(S09):Induce protein and evaluate DNA (Day5): Difference between revisions
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| No | | No | ||
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| | |Red | ||
| | |0.121 | ||
| | |0.187 | ||
| | |0.133 | ||
| | |Yes | ||
|- | |- | ||
| | | |
Revision as of 14:56, 24 February 2009
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/c8/20.109_induced-pellets.jpg/500px-20.109_induced-pellets.jpg)
TR O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
Group | OD WT | OD S101L | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Pink | 0.117 | 0.098 | 0.126 | Yes |
Yellow | No finished samples | N/A | N/A | No |
Red | 0.121 | 0.187 | 0.133 | Yes |
Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V.
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/6/61/S09_M1D3_TR_Yellow.BMP/250px-S09_M1D3_TR_Yellow.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/7f/S09_M1D3_TR_Orange.BMP/250px-S09_M1D3_TR_Orange.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/0/0d/S09_M1D3_TR_Blue.BMP/250px-S09_M1D3_TR_Blue.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/f2/S09_M1D3_TR_Grey.BMP/250px-S09_M1D3_TR_Grey.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/d/d1/S09_M1D3_TR_Red.BMP/250px-S09_M1D3_TR_Red.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/49/S09_M1D3_TR_Pink.BMP/250px-S09_M1D3_TR_Pink.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/9/96/S09-M1D5_teaching-ex.BMP/250px-S09-M1D5_teaching-ex.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/c8/S09_M1D3_TR_Green.BMP/250px-S09_M1D3_TR_Green.BMP.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/5/56/S09_M1D3_TR_Purple.BMP/250px-S09_M1D3_TR_Purple.BMP.png)
There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which is posted above. Unfortunately, some of the bands are faint. Lane 1 is parent IPC (only one form of the plasmid is visible), lane 2 is the ladder, lane 4 is the parent digested with FspI, and lane 8 is the S101L mutant digested with FspI. The short band is very faint, but it is present. I will have the original photo available for viewing for anyone who wants to clearly see the short band.
WF O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
Group | OD WT | OD Mut1 | OD Mut2 | Pellets yellow? |
---|---|---|---|---|
pics here