User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/10: Difference between revisions
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* | * Lenti Transfection: Day 2 - change out medium (LaBaer lab) | ||
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''' | '''Lenti Transfection: Day 2 - change out medium'''<br> | ||
Note: At this point, cells are producing virus. Follow extra biosafety precautions. | |||
* Medium change 1 - 10:00 am | |||
# PPE note: Wear 2x pairs of gloves. Dispose of and replace outer gloves as needed. | |||
# In the BS cabinet, set up 2x 50 mL conicals of 10 mL Wescodyne (brown) germicidal reagent. Use one for discarding viral medium, one for rinsing pipettes. | |||
# Checked GFP control (sample 7) under scope. Nearly 100% expression. (Note: scope had no RFP filter set) | |||
# For each well (one at a time)... | |||
## '''Removed 1.5 mL growth medium''', dispensed into Wescodyne, rinsed pipette in Wesc., discarded pipette | |||
## Slowly and carefully '''added 2 mL fresh growth medium''', rinsed pipette in Wesc., discarded pipette | |||
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Revision as of 12:43, 10 September 2014
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09/10/14
Lenti Transfection: Day 2 - change out medium
--> Reaction conditions
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