Haynes Lab:Notebook/Engineering PC-TFs/2014/06/09: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 15: | Line 15: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. Unknown <br> | | rowspan="7" | <u>Expected:</u><br>1. Unknown <br><u>Observed:</u><br> 3k, 7k, 2k<br> | ||
|- | |- | ||
| DNA(CMV/MV9) || 5.0 μL | | DNA(CMV/MV9) || 5.0 μL | ||
Line 35: | Line 35: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. 5200bp <br> | | rowspan="7" | <u>Expected:</u><br>1. 5200bp <br><u>Observed:</u><br> 5.2k, 2k<br> | ||
|- | |- | ||
| DNA(CMV/MV9) || 5.0 μL | | DNA(CMV/MV9) || 5.0 μL | ||
Line 55: | Line 55: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. 5200bp <br> | | rowspan="7" | <u>Expected:</u><br>1. 5200bp <br><u>Observed:</u><br> 5.2k, 2k<br> | ||
|- | |- | ||
| DNA(CMV/MV9) || 5.0 μL | | DNA(CMV/MV9) || 5.0 μL | ||
Line 75: | Line 75: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. 710bp, 4490bp <br> | | rowspan="7" | <u>Expected:</u><br>1. 710bp, 4490bp <br><u>Observed:</u><br> 4k, 2k, 700<br> | ||
|- | |- | ||
| DNA(CMV/MV9) || 5.0 μL | | DNA(CMV/MV9) || 5.0 μL | ||
Line 94: | Line 94: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. BL01 = 2520bp, V0120 | | rowspan="7" | <u>Expected:</u><br>1. BL01 = Insert (2520bp), V0120(3265bp) <br><u>Observed:</u><br> 3.1k, 2.6k<br> | ||
|- | |- | ||
| DNA(BL01A) || 5.0 μL | | DNA(BL01A) || 5.0 μL | ||
Line 113: | Line 113: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. BL01 = 2520bp, | | rowspan="7" | <u>Expected:</u><br>1. BL01 = 2520bp, 3265bp <br> <u>Observed:</u><br> 3.1k, 2.6k, 7k<br> | ||
|- | |- | ||
| DNA(BL01B) || 5.0 μL | | DNA(BL01B) || 5.0 μL | ||
Line 132: | Line 132: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br> | | rowspan="7" | <u>Expected:</u><br> 2520bp, 3265bp <u>Observed:</u><br> Faint: 3.1k, 2.6k, 7k<br> | ||
|- | |- | ||
| DNA(BL05) || 5.0 μL | | DNA(BL05) || 5.0 μL | ||
Line 151: | Line 151: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. | | rowspan="7" | <u>Expected:</u><br> 1431bp, 3265bp <u>Observed:</u><br> 3.1k, 4.9k, 1.5k <br> | ||
|- | |- | ||
| DNA(BL09) || 5.0 μL | | DNA(BL09) || 5.0 μL | ||
Line 170: | Line 170: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. BL01 = 7720bp <br> | | rowspan="7" | <u>Expected:</u><br>1. BL01 = 7720bp <br> <u>Observed:</u><br> 4k, 2k, 1.5k <br> | ||
|- | |- | ||
| DNA(CMV/MV9 & BL01) || 5.0 μL | | DNA(CMV/MV9 & BL01) || 5.0 μL | ||
Line 189: | Line 189: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. dependent on ligation direction <br> | | rowspan="7" | <u>Expected:</u><br>1. dependent on ligation direction <br><u>Observed:</u><br>4k, 2k, 1.5k<br> | ||
|- | |- | ||
| DNA(CMV/MV9 & BL01) || 5.0 μL | | DNA(CMV/MV9 & BL01) || 5.0 μL | ||
Line 208: | Line 208: | ||
{| {{table}} border="1" cellspacing="3" <!-- Plates. table --> | {| {{table}} border="1" cellspacing="3" <!-- Plates. table --> | ||
|- valign="top" | |- valign="top" | ||
| rowspan="7" | [[Image:20140609 172345.jpg|400px|Hover name]] <br> | | rowspan="7" | [[Image:20140609 172345.jpg|400px|Hover name]] <br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|} | |} | ||
Latest revision as of 00:02, 27 September 2017
Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SummaryDigest All the Things Well One: Ladder
Well Four
Gel Verification
|