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Purification and ligation

  • Today we purified our digested vector, as well as our PCR results that were cut out of the gel from last time.
  • We ran a sample of the purified vector and DNA on a gel to analyze the concentrations.
  • After analyzing the concentrations of the vector and the DNA, we then set up a series of ligations of different concentrations. This will allow our DNA peice to become included in the vector. Our next step is to transform some cells with our ligations to see if it worked.