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Restriction Digest and gel

  • Some of our mini prep was used for a restriction digest. Restriction enzyms were chosen that would digest all of our plasmids so that we could run a gel and get plasmids and parts all separated out by lenght.
  • We then ran a gel that had already been made the previous week by another group.
  • Turns out, that fresh gels should be used. The gel was bad, and we have to redo this day's experiment on tuesday.