From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png MoldBusters' Journal Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Tuesday 3/24

Josh and Casy

  • The DNA samples stored in isopropanol were centrifuged, washed with ethanol, and dried. Dissolved in 20μL of water.
  • A gel was ran with 2μL of DNA from each sample. Of the four samples, the first two were ran undigested. The other two were digested with EcoR1.
  • The digested DNA was fragmented, indicating that the samples did contain DNA. However, no comparison could be made as to the purity of the undigested samples.


  • Dissolved nprE primers in U.V. treated H2O to achieve a concentration of 100μM
  • Stored oligos at -20°C