Josh and Casy
- The DNA samples stored in isopropanol were centrifuged, washed with ethanol, and dried. Dissolved in 20μL of water.
- A gel was ran with 2μL of DNA from each sample. Of the four samples, the first two were ran undigested. The other two were digested with EcoR1.
- The digested DNA was fragmented, indicating that the samples did contain DNA. However, no comparison could be made as to the purity of the undigested samples.
- Dissolved nprE primers in U.V. treated H2O to achieve a concentration of 100μM
- Stored oligos at -20°C