840:153g:Projects/project4/2009/03/24

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Tuesday 3/24

Josh and Casy

  • The DNA samples stored in isopropanol were centrifuged, washed with ethanol, and dried. Dissolved in 20μL of water.
  • A gel was ran with 2μL of DNA from each sample. Of the four samples, the first two were ran undigested. The other two were digested with EcoR1.
  • The digested DNA was fragmented, indicating that the samples did contain DNA. However, no comparison could be made as to the purity of the undigested samples.

Oggie

  • Dissolved nprE primers in U.V. treated H2O to achieve a concentration of 100μM
  • Stored oligos at -20°C