840:153g:Projects/project4/2009/02/09

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Monday 2/9

Josh and Casy

  • The second transformation yielded the same results as previously, with only the transformants of R0080 plasmid from 2007 growing. Due to the higher concentration of DNA used for this transformation, many more transformants were obtained.
  • Five colonies from each of these plates were inoculated onto ampicillin plates.
  • The plates containing the five transformed colonies grew as expected, and were used to inoculate five liquid cultures (containing ampicillin) to be grown overnight.
  • Two liquid cultures were inoculated with each type of B. subtilis.