840:153g:Projects/project4/2009/01/29

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Thursday 1/29

Josh and Casy

  • Extracted vector BBa_R0080 and BBa_I0500 from the Biobrick Library from both 2007 and 2008.
  • The extractions from the 2007 library were stored in the freezer, and those from the 2008 library were stored at room temperature on the lab bench.
  • The procedures from each kit were followed, with modifications to the volumes used.
  • These should be ready to use in transformation.

Derek, Katy, and Oggie

  • We made LB medium, and autoclaved our solution. However, we forgot to add the agar. We will be waiting till a later date to add the agar - and re-autoclave.

Derek

  • Found new protocols for DNA extaction.