April 23, 2009
- We ran a gel to see the results of our RT-PCR that we performed last Tuesday using two different temperatures: 46 and 50 degrees Celsius for all the samples. PCR worked because our internal positive control (HOPS) worked and showed bands around 400 and 250 basepairs. However, our primers didn't work because we see bands in every well at 400 basepairs. This indicates our primer didn't work because every sample shouldn't be the same and there is no amplified RNA.