840:153g:Projects/project3/2009/04/21

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April 21, 2009

  • Today we ran RT-PCR on our two forward primers using two different temperature gradients (46, 50 degrees Celsius). We are changing the annealing time also from 1 minute to two minutes. Otherwise it will be the same procedure as last week. For Thursday we will run a gel to confirm results.
  • We also worked on our presentation.