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March 24, 2009

  • Today we ran a 8% agarose gel to confirm PCR results. Unfortunately we were unable to obtain good results so we are start over with PCR next week. For Thursday we are going to design new primers (using out old ones but without the extensions). Once the new primers come in we will run PCR and see if they work. We will also modify our PCR by running more DNA (10 microliters) in our PCR samples at three different temperatures and by diluting our DNA at those same temperatures. This will determine if the amount of DNA is the problem in our PCR.
  • We also extracted RNA out of the the onion tissue using the RNEasy RNA kit. We then prepared the electrophoresis equipment for our RNA gel we will be running Thursday.