840:153g:Projects/project3/2009/03/03

Tear Inducing Bacteria

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March 3, 2009 We ran a gel to confirm that high molecular DNA was extracted from our onion. We had 8 DNA samples that were combined into one tube and 10 microliters of RNase A was added. PCR was then run using our two different primers and 5 different gradients. The next step is to run a gel to confirm PCR worked on Thursday. We also ran a gel for our plasmid DNA to confirm that we had the correct plasmids. We digested the DNA with Xba I and Pst I. Our gel was facing the wrong direction and so it did not run correctly. Therefore, we will have to redo this procedure on Thursday.