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Plasmid Extraction from Transformed competent cells

  • Plasmid extraction from overnight incubated transformed e.coli plates

Agarose gel electrophoresis (PCR: 2nd Attempt)

  • Results very familiar to 1st attempt of PCR.

PCR(3rd Attempt

  • On the results on 2nd PCR attempt.We decide to next gradient pcr for our nprT Gene at higher temperature(above 48 degree Celsius)