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The Sweet Smell of.....E.coli?
Growth of colonies are observed on all the plates that was streaked yesterday. A slight concern for part J45120 as the colonies appear light blue while all other parts has white colonies. Several reasons could account for this, one is that the plasmids do not contain the insert or that the inserted DNA is in the right reading frame that allowed the lac Z region to also be expressed. The presence of the DNA insert in the plasmid will be tested in class tomorrow using gel electrophoresis. Aaron prepared overnight cultures in 5ml LB+ Ampicillin.
Updated by: Diveena