YPD and YPAD medium

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  • Omit agar for liquid culture.
  • Agar plates are at 2% rather than the 1.5% usual in bacterial work.
  • Dry the plates 2-3 days at RT before storing at 4C for several months.
  • Autoclave components for a maximun of 15 minutes at 121C.
  • For large volumes, autoclave salts, glucose and agar separately.


YPD (YEPD) medium

A complex medium for routine growth. Available premixed as Difco 0428-17.

  • per liter
  • Bacto yeast extract (Difco 0127-17) (1%) 10g
  • Bacto peptone (Difco 0118-17) (2%) 20g
  • Glucose (2%) 20g
  • Bacto agar (Difco 0140-01) (2%) 20g


YPAD medium

A complex medium to inhibit reversion of the ade1 and ade2 mutations. As above, with added adenine. Inhibits red coloration in ade strains such as AB1380.


  • Bacto yeast extract (Difco 0127-17) (1%) 10g
  • Bacto peptone (Difco 0118-17) (2%) 20g
  • Glucose (2%) 20g
  • Bacto agar (Difco 0140-01) (2%) 20g
  • Adenine sulfate (.004%) 40mg


YPG (YPG or YEP-glycerol) medium

A complex medium containing non-fermentable carbon source (glycerol) that does not allow growth of ρ- or pet mutants.

  • per liter
  • Bacto yeast extract (Difco 0127-17) (1%) 10g
  • Bacto peptone (Difco 0118-17) (2%) 20g
  • Glycerol (3% v/v) 30 ml
  • Bacto agar (Difco 0140-01) (2%) 20g
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