WinterVomitingLab:Protocols/Stool suspension

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Contents

Overview

Preparation of a 10-20% suspension of virus stock from a stool (or vomit) sample.

Materials

  • PBS
  • stool or vomit

Procedure

  1. To 2 ml screw-cap tubes, add 750 µl of PBS.
  2. If the sample is solid or semi-solid, add a pea-sized (~0.1 g) of stool to the PBS. If it is mostly liquid and can be pipetted, add 0.1 ml to the PBS.
  3. Vortex thoroughly for ~ 1 min.
  4. Centrifuge for 10 min at 10,000 rpm in a microcentrifuge.
  5. Collect the stool suspension (supernatant). Note: the pellet can be retained and re-extracted later as it likely will still contain high levels of virus.
  6. Stool suspensions can be used immediately or otherwise stored (in aliquots) at -70°C.


References

Relevant papers and books

  1. []

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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