# Winning Lab Report Criteria

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Scholars,

Here is a recipe for a winning module 3 report:

- Microscope documentation
- Microscope block diagram
- All optical elements and distances
- It is not necessary to document mechanical construction details
- A picture of the microscope is nice

- Microscope block diagram

- Characterization
- Table of values for 10x, 40x, and 100x objectives, including
- Theoretical magnification
- Measured magnificaiton
- Theoretical resolution
- Field of view (diagonal)

- Bright field images
- Air Force Target, Ronchi Ruling, 4 micron latex spheres
- 10/40/100x objectives

- Table of values for 10x, 40x, and 100x objectives, including

- Fluorescence microscopy
- Image of fluorescence calibration slide (for flat field correction)
- Optional: flat field corrected image of 1 micron spheres
- Combined onion image
- Bright field onion image with flat field corrected, fluorescent image (color) overlayed
- Bullet point overview of processing algorithm used to produce image

- PSF
- PSF image (.190 micron beads) – preferably zoomed in to a few particles
- Estimated ½ intensity width of beads
- Was it the same in both directions?

- Particle tracking
- Dry beads
- X-Y plot of particle tracks and differential track
- Plot of MSD vs. delta t

- Brownian motion (1 and 2 micron beads in suspension)
- X-Y plots of particle tracks (particles in different colors)
- MSD vs. delta time plots with ensemble average
- Estimated diffusion coefficients
- Extra credit: estimate Avagadro’s number (see: http://www3.interscience.wiley.com/cgi-bin/abstract/109884431/ and http://www.math.princeton.edu/~mcmillen/molbio/papers/Einstein_diffusion1905.pdf)

- Transport in an onion cell
- X-Y plot of particle tracks
- MSD vs delta t plot
- Particle velocity: average, deviation
- Extra credit: estimate the number of myosin motors per granule

- Dry beads

--Steve