WangLab:Simple Purification of Adenovirus
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Simple purification of adenovirus
- 50 ul to DMEM 10 ml
- Put into preconfluent 293 cells.
- 24 hr later, see green
- 48 hr later, floating cells.
- Collect cell+sup
- Freeze-thaw 3 times (no warming)
- Centrifuge 1000g
- Get supernatant
- Aliquote and store in -70.
- 100-200 ul to 10 ml DMEM.