WangLab:Ligation of Reporter to Adenovirus Ad5

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Ligation of reporter to Adenovirus Ad5

  • A+B ligation
A B
1. Cut Src reporter with BamHI/EcoRI 1. pTet cut with EcoRV
2. Purify 2. Purify and dephosphorylate with CIAP.
3. Klenow +dNTP to fill at 70oC, 15 min 3. Phenol/chlorut purify
4. Get the insert. 4. Get the vector.
Retrieved from "http://openwetware.org/wiki/WangLab:Ligation_of_Reporter_to_Adenovirus_Ad5"
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Contributing Authors
  • D. Knipmeyer