WangLab:Ligation Samples

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Ligation Samples

Two piece ligation

  • 5X ligation buffer: 4
  • Vector: 1
  • Insert: 3
  • T4 ligase: 1
  • H2O: 11

Three piece ligation

  • 5X ligation buffer: 4
  • Vector: 1
  • Insert 1: 5
  • Insert 2: 5
  • T4 ligase: 1
  • H2O: 4
Retrieved from "http://openwetware.org/wiki/WangLab:Ligation_Samples"
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