WangLab:HEPES buffer

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HEPES

  • 4-(2-Hydroxyethyl)-1-piperazineethanesuffonic acid
  • C8H18N2O4S
  • Molecular weight = 238.30

HEPES buffer

  • 20mM HEPES buffer, PH=7.3
  • x/(238.30*0.1)*10^3 = 20
  • x = 0.4766g
  • 100ml H2O
  • 1mol/L NaOH
  • x’/(40*0.1) = 1.0
  • x’ = 4g
  • 2g : 50ml H2O, 1g, 25ml H2O
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