User talk:Anthony Salvagno/Notebook/Research/2009/11/11/Andy's Poster

Steve Koch 22:20, 11 November 2009 (EST): These look great! I love the casein diagram, though I don't know enough to comment on accuracy. I also love the kinesin diagram and it is definitely what I want to use in my own talks, etc. My main worry is being misleading about heavy water being bigger. The density of heavy water is actually higher than regular water (by 10%; thus "heavy"). I'm not sure if there's anything you can draw to illustrate the bulk differences, except maybe coloring the hydrogens and deuteriums differently. As I've discovered in the past couple weeks, there are all kinds of things that go on in D2O, including D exchange with exposed H's on the proteins. We'd probably want to figure out how to draw that too. Interestingly, Oxygen-18 water (versus 16) is more simple, because it's still hydrogens, so you don't have to worry about the exchange.

The drawing can easily be adapted for high osmotic stress, in which case you'd delete a few water molecules for some other molecules (lower concentration of water).

Anthony Salvagno 01:40, 12 November 2009 (EST):Yea I didn't really know what the difference was, but I couldn't think of a way to show more massive outside of making it bigger. Bigger molecules can obviously demonstrate different properties. I originally wanted to make the kinesin head look more like the kinesin from the KochLab logo I made a while back, and can adapt it to that pretty easily if you don't want the kiney feet.

Steve Koch 02:27, 12 November 2009 (EST): Yeah I hear you, it is tough to convey. But I'm still worried about misleading a casual reader of the poster. So, coloring the H versus D differently may be the easiest way to go. Here's a paper which gives a great overview of the variety of things that D2O can do to biological systems. Read the introduction, "The diversity of D2O effects on biological systems": File:PNAS (1973) Pittendrigh et al Heavy Water effect on circadian rhythm.pdf

I like the shoes.

Steve Koch 02:29, 12 November 2009 (EST): Also, you could put a few more ordered water molecules around the molecular surfaces, to indicate their hydrating water molecules.