March 18th
- Use alpha and gamma methods to make myoglobin GNPs
Procedure
Alpha
- Dilute 50 mg/mL myoglobin to 10^-6 M
Gamma
- A 1/10 solution of the reactant free nano particles was prepared from the stock solution
- 27mL of the AuNP solution was transferred to a beaker
- 1mL of 5mg/mL solution Myoglobin in H2O was added to the beaker
- The mixture was stirred at room temperature for 30 minutes
- The solution was then centrifuged for 30 minutes at 10,000rpm
- The supernatant and pellet were separated
- The pellet was resuspended in PBS solution with 0.1% BSA
Notes
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