User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/08

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Summary

  • run 1x 6% SDS-PAGE (prepared 1.10.2010) (2nd gel had bad comb)
6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates 		X Fermantas high range Marker
(10 μL) 		Jurkat
(30 μL) 		HEK293
(30 μL) 		HeLa -S
(30 μL) 		Fermantas high range Marker
(10 μL) 		S0
(30 μL) 		S1
(30 μL) 		X S2
(30 μL) 		S3
(30 μL) 		C0
(30 μL) 		C1
(30 μL) 		C2
(30 μL) 		C3
(30 μL)
  • Run at 200 V
  • Prepared
    • Fixing solution (Coomassie)
      • 40% EtOH
      • 10% Acetic acid
    • Destain solution (Coomassie)
    • 10% Acetic Acid
      • NOTE: Could only find Glacial acetic acid (anhydrous acetic acid)


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