User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/16
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To look at the effect of Protein kinase A (PKA) and Epac on the IL-8 production by airway smooth muscle cells (ASMC's) induced by cigarette smoke extract (CSE), 8-pcpt-2'-o-me-camp (8-pcpt) and 6-Benz-cAMP (6-Benz) are used to activate Epac and PKA respectively. To illustrate the influence of A kinase Anchoring Proteins (AKAP's) an allround inhibitor of PKA (subunit RII) binding to AKAP's HT31 is used. An inactive peptide HT31P is used as an control.
Materials & Methods
CTR = control, CSE = Cigarette Smoke extract, 8-pcpt = 8-pcpt-2'-o-me-camp
Run 1: Ht31/Ht31P D9/D12