User:Vijay/Notebook/2007-2-12

Fusion

1. Plasmid prep from phoA/lacZ (pBAF/pLKC480) and gel. Done
2. Double digest phoA/lacZ and gel. Done
   1. 8 μl vector
   2. 2 μl buffer
   3. 1 μl enzyme
      • EcoR1 for 1hr at 37⁰C, followed by Sal1 for 1hr at 37⁰C in cycler
   4. 9 μl water
3. Stock phoA/lacZ. Done

Fractionation

1. Inoculate UAMS-1 wt for 2nd fractionation. Done