User:Torsten Waldminghaus/Notebook/Multiple Mutation Reaction/2008/04/28
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Two 50 μL reaktions with all primers and one PCR control with only the flanking primers:
5 min at 95°C
Note: we have a quite old PCR machine and I had problems programming it so that the samples incubated at 65 °C for about 2 hours. I than started the right program and left it over night at 4 °C in the machine.