User:Tk/Notebook/MF/2009/02/27

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MF Recombination Cassettes

  • Required parts
    • constitutive promoter: possibly use the B. subtilis constitutive (K143012)
    • CIRCE element promoter
    • CAT cassette (J70026)
    • PheS mutant gene
    • modified CFP
    • MF deletion region homology parts
    • ME end parts (M1000; M1001)
  • optional/next parts
    • recE
    • recT
    • YFP
    • RFP
    • plasmid backbone (J70027)


  • Transposon constructs, made with insertions into existing TT01 part
    • const promoter + CFP
    • CIRCE + CFP
    • cat cassette; PheS cassette in same construct


  • recombination parts
    • transposon insertion of CIRCE + recT + recE
    • homology - cat cassette - pheS cassette - homology
      • do we need thio primers for PCR of this?
    • homology - homology for testing deletion


  • plasmid tests
    • plasmid + cat cassette (J70027 + J70026)
    • plamid + constitutive + CFP




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