User:Timothy L. Foley/Notebook/refolding matrix/2009/10/13/Refolding Matrix-10-13-2009 amino acids 4-factor

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buffer # pH detergent amino acids salt crowders divalent cation GnCl BSA Ligand reducing agent Amino acid
1 5.5 MES none none low none 1 0 1 1 none Arg
2 5.5 MES none none high Glycerol 0 1 1 1 TCEP Glu
3 5.5 MES none Glut none none 0 0 0 0 none Arg+Glu
4 5.5 MES none Glut low Glycerol 0 0 0 0 TCEP none
5 5.5 MES TritonX none high none 1 0 0 1 none Glu
6 5.5 MES TritonX Arg low PEG 1 1 1 1 none Arg+Glu
7 5.5 MES TritonX Arg low Glycerol 0 1 0 0 TCEP Arg
8 5.5 MES TritonX Arg high Glycerol 1 1 1 0 none Arg+Glu
9 5.5 MES TritonX Glut none none 1 0 1 0 TCEP Arg+Glu
10 5.5 MES Tween80 none none PEG 0 1 1 1 DTT none
11 5.5 MES Tween80 none none Glycerol 1 1 0 0 DTT Arg
12 5.5 MES Tween80 Arg none PEG 0 0 0 1 TCEP Arg
13 5.5 MES Tween80 Arg low Glycerol 0 0 0 0 none Glu
14 5.5 MES Tween80 Arg high none 1 1 1 1 DTT Arg
15 5.5 MES CHAPS none high Glycerol 0 0 0 0 DTT Arg+Glu
16 5.5 MES CHAPS Arg none none 1 0 0 1 TCEP none
17 5.5 MES CHAPS Glut none PEG 0 1 0 1 none Glu
18 5.5 MES CHAPS Glut low PEG 1 1 1 0 DTT Glu
19 5.5 MES CHAPS Glut high PEG 1 1 1 0 DTT none
20 6.5 MES none none none none 1 1 0 0 none Arg
21 6.5 MES none none low Glycerol 1 0 0 1 DTT none
22 6.5 MES none Arg none PEG 1 0 0 1 DTT none
23 6.5 MES none Glut high none 0 1 1 0 none Arg+Glu
24 6.5 MES none Glut high PEG 0 1 0 0 TCEP Arg+Glu
25 6.5 MES TritonX none low none 0 1 0 0 TCEP none
26 6.5 MES TritonX none high Glycerol 1 1 1 1 DTT Arg
27 6.5 MES TritonX Arg none none 0 1 0 0 none Glu
28 6.5 MES TritonX Arg high PEG 1 0 1 1 DTT Arg+Glu
29 6.5 MES TritonX Glut low Glycerol 1 0 1 1 none Arg
30 6.5 MES Tween80 Arg none none 0 1 1 1 DTT Arg
31 6.5 MES Tween80 Arg none Glycerol 0 0 1 1 none Arg+Glu
32 6.5 MES Tween80 Glut low PEG 1 1 0 0 TCEP Glu
33 6.5 MES Tween80 Glut low Glycerol 1 1 1 1 TCEP none
34 6.5 MES Tween80 Glut high Glycerol 0 1 1 1 none Glu
35 6.5 MES CHAPS none none none 0 0 1 0 TCEP Arg
36 6.5 MES CHAPS none low PEG 0 0 1 0 none Glu
37 6.5 MES CHAPS none low Glycerol 1 0 0 0 TCEP Arg+Glu
38 6.5 MES CHAPS Arg high PEG 0 0 0 1 TCEP none
39 6.5 MES CHAPS Arg high PEG 1 0 0 0 DTT Glu
40 7.5 HEPES none none high none 1 0 0 1 TCEP Glu
41 7.5 HEPES none Arg none Glycerol 0 0 1 0 DTT Glu
42 7.5 HEPES none Glut none Glycerol 1 1 1 1 TCEP Arg+Glu
43 7.5 HEPES none Glut low none 0 0 1 1 DTT none
44 7.5 HEPES none Glut low PEG 0 0 0 1 DTT Arg
45 7.5 HEPES TritonX none none PEG 1 1 1 0 DTT Glu
46 7.5 HEPES TritonX none low none 0 1 0 1 DTT Glu
47 7.5 HEPES TritonX none high PEG 0 0 1 1 none Arg
48 7.5 HEPES TritonX Arg none PEG 0 0 1 0 none none
49 7.5 HEPES TritonX Glut high Glycerol 0 0 0 0 TCEP Glu
50 7.5 HEPES Tween80 none low PEG 1 0 1 1 TCEP Arg+Glu
51 7.5 HEPES Tween80 none high Glycerol 1 0 1 0 TCEP Arg+Glu
52 7.5 HEPES Tween80 Arg high none 1 0 0 0 DTT none
53 7.5 HEPES Tween80 Glut none PEG 1 1 0 0 TCEP Arg+Glu
54 7.5 HEPES Tween80 Glut high none 1 1 0 0 none Arg
55 7.5 HEPES CHAPS none none none 0 1 1 1 none none
56 7.5 HEPES CHAPS Arg none Glycerol 1 1 0 0 none none
57 7.5 HEPES CHAPS Arg low none 0 1 1 0 DTT Arg+Glu
58 7.5 HEPES CHAPS Arg low Glycerol 1 1 0 1 TCEP Arg
59 7.5 HEPES CHAPS Glut high Glycerol 0 1 0 1 none Arg
60 8.2 Tris none none none PEG 0 1 0 0 TCEP Arg
61 8.2 Tris none none none Glycerol 0 1 1 0 none none
62 8.2 Tris none Arg high none 1 1 1 1 TCEP Glu
63 8.2 Tris none Arg high PEG 0 1 0 1 none Arg+Glu
64 8.2 Tris none Arg high PEG 1 0 0 0 DTT Arg
65 8.2 Tris TritonX none low PEG 0 0 0 1 none Arg+Glu
66 8.2 Tris TritonX Glut none PEG 1 1 1 0 TCEP Arg
67 8.2 Tris TritonX Glut none Glycerol 1 0 1 1 TCEP none
68 8.2 Tris TritonX Glut low none 1 1 0 0 DTT none
69 8.2 Tris TritonX Glut high Glycerol 0 0 0 1 DTT none
70 8.2 Tris Tween80 none low none 0 0 1 0 DTT Arg
71 8.2 Tris Tween80 none high none 0 0 0 0 none none
72 8.2 Tris Tween80 Arg low PEG 0 1 1 1 TCEP Glu
73 8.2 Tris Tween80 Arg high none 1 0 1 0 TCEP Arg+Glu
74 8.2 Tris CHAPS none none Glycerol 1 1 0 1 DTT Arg+Glu
75 8.2 Tris CHAPS Arg low Glycerol 0 1 1 0 DTT Glu
76 8.2 Tris CHAPS Glut none none 1 0 1 1 none Glu
77 8.2 Tris CHAPS Glut low Glycerol 1 0 0 1 none Arg
78 9.2 CHES none none none Glycerol 1 0 1 0 DTT Glu
79 9.2 CHES none Arg low none 0 1 0 1 TCEP Arg+Glu
80 9.2 CHES none Arg low PEG 1 0 1 0 none Arg
81 9.2 CHES none Arg low Glycerol 1 1 1 0 none none
82 9.2 CHES none Glut high none 1 1 1 1 DTT Glu
83 9.2 CHES TritonX none high Glycerol 0 1 1 0 TCEP none
84 9.2 CHES TritonX Arg none none 1 0 0 1 TCEP Glu
85 9.2 CHES TritonX Arg high Glycerol 0 1 0 0 DTT Arg
86 9.2 CHES TritonX Glut none PEG 0 1 0 1 DTT Arg+Glu
87 9.2 CHES Tween80 none low PEG 1 1 0 1 none none
88 9.2 CHES Tween80 none high PEG 1 1 0 0 none Glu
89 9.2 CHES Tween80 Arg none Glycerol 1 0 0 1 none Glu
90 9.2 CHES Tween80 Glut none Glycerol 0 0 0 1 DTT Arg+Glu
91 9.2 CHES Tween80 Glut low none 0 0 1 0 DTT Arg+Glu
92 9.2 CHES CHAPS none low none 1 1 0 1 DTT Arg+Glu
93 9.2 CHES CHAPS none high PEG 0 0 1 1 TCEP Arg
94 9.2 CHES CHAPS Arg high none 0 1 1 1 TCEP none
95 9.2 CHES CHAPS Glut none none 0 0 1 0 TCEP Arg
96 9.2 CHES CHAPS Glut high PEG 1 0 1 0 none none