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Gaussian Point Spread Function
So my point spread function will be approximated as a Gaussian. It can be an Airy Disc but i still don't know how to make a 3-d airy disc, so for now I'll use a Gaussian.
After today's group meeting Koch helped me out with some troublesome parts of this simulation
Right now i have the Gaussian spread to be about .09 which looks pretty good but i am unhappy with this procedure there must be a more precise way to do this. I just don't know how, and every where i look this part seems glazed over
On the bright side i got my 3-d Airy Disc going. Hooray for me. Now i can use this instead of the Gaussian for my point spread function. That is good news.
This opens up the question now of how do i deal with the smear. before i didn't know what i was doing but was confident i know what sigma of the Gaussian is. Now I have this Airy Disc and I am not 100% confident i know what to change to match experimental conditions. Here i'll write down what i do know:
Oh so i don't forget Andy said there are probably some software packages that figures out the PSF already. That might be worth looking into if this starts to drive me crazy. But i feel right now that i am on the right path.
I'll fight it but i probably have to read a book or a chapter or something to get this right.
OK I'll read Born/Wolf chapter on Airy Discs. Chapter 8. Hopefully that gives me some background to play with this function. I am keeping my fingers crossed that the wavelength and aperture size is all i really need to match the images from the microscope. It could be -- i wanna believe (also I0)
Also if any one knows this aperture size please feel free to put it here