SCOC Multiplex Optimization
PCR
SCOC MPY
- Problem: marker ranges in yellow lane of SCOC Multiplex may be wider than currently set (particularly Scun11)
- Experiment: run Scun3, Scun11 and sar80 in uniplex on wide range of samples to establish marker ranges
SCOC MPG
- Problem: Scun19 showing up low intensity
- Experiment: decrease tall Scun15, increase short Scun19 to even out peak heights
SCPC MPG + sar80
- Problem: sar80 seems to want to be run in uniplex because it doesn't play well with others in the sandbox
- Experiment: try to stick sar80 primers into MPG (same buffer and annealing temperature as MPY) to prevent having to run sar80 in uniplex always
CNHY Multiplex Optimization
PCR
- start trying to group markers together into multiplexes
- start with same volume of each primer (can modify primer volumes based on results of this PCR)
CNHY MPA
- Ai5033, Ai5057, Ai5037, Acos5, Ai5035, Ai5062
CNHY MPB
- Ai5043, Acos3, Ai5013, Ai5071
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