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CNHY / CNTI Multiplex
PCR - Uniplexes
- uniplex to determine marker range
- individual CNHY and CNTI DNAs to determine marker quality when uniplexed
- pooled CNHY and CNTI DNAs to determine marker range when uniplexed
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PCR - Multiplex all-in-one
- multiplex to determine general marker quality and strength
- individual CNHY and CNTI DNAs to determine marker quality when multiplexed
- pooled CNHY and CNTI DNAs to determine marker range when multiplexed
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PCR - Multiplex A and Multiplex B
- split multiplex into two (no post-PCR mixing) based on primer interactions, gradient results, and estimated amplicon size
- compare all-in-one multiplex with Multiplex A and B - better?
- compare scores in multiplex A and B with uniplex to make sure scores match and nothing weird is happening
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Tara's Lab Notebook
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