User:Tara K. Luckau/Notebook/Team ConGen/2011/11/01

SCOC MP1

Gel

• (used piece of gel that was poured 12 September)
• run H1-6 (includes H₂O) for all three PCR plates

• not all loci are apparent, but PCR did work, so send to frag

absent bands will hopefully be smaller peaks on pherogram

Ship and Fragment Analysis Submission

• FedEx Tracking# 876927708718

• UAGC Submission# AXRJ

• FedEx picked up 2:56pm, 1 November
• FedEx delivered 9:15am, 2 November
• UAGC received 12:26pm, 2 November
• UAGC completed 10:08am, 3 November

Meeting with Rulon

Proposal Stuff

• feasibility of getting enough SCOC data as well as enough CNTI data ... nope, not gunna happen
• discussed possibility of pushing proposal to next semester (March)
• Arguments to propose in December:

1. proof of concept will, actually, be possible
2. proof of concept = molecular techniques work (and Tara is capable of doing them), fine-scale structure is detectable with these techniques, and these species would be a cool comparison
3. I'm confident I'll be able to get enough samples of SCOC run and analyzed for the proposal in December (but whiptails will not get there)
4. because a proposal should be just that - a proposal

• Arguments to propose in March:

1. the precedent set for proposals has been much more than a proposal - it's kinda like a pre-defense
2. will have more time to get whiptails up and running
3. may be able to validate some of the markers using Andy's suggestion of genotyping a family group (related individuals)

• will talk with Andy to see his thoughts ...