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SCOC MP1
- First time running multiplex within lanes
- First time post-PCR mixing (1:1:1)
- First time sub-sampling across San Diego county, focusing on RAJ/HOL and SYR
- will send individual MP plates (blue, green, yellow) to Tucson, in case mixing doesn't work out
- UAGC may be able to play with ratios
- UAGC will be able to run one color at a time, if needed
PCR
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- oops - mispipetted (dispensed, but aspirated before pulling tip out of PCR plate) SCOC RAJ 49, redispensed into SCOC RAJ 48
- SCOC RAJ 48 may be contaminated with SCOC RAJ 49
- SCOC RAJ 49 may have low amount of template DNA
- had lots of left-over master mix ... decrease to 5% overage (from 10% overage) to save on reagent cost
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- still had lots of left-over master mix! could decrease even more, to 3% overage
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- still had lots of left-over master mix! could decrease even more, to 3% overage
1Kb Ladder
- made two aliquots of 1Kb ladder, according to protocol
- 120µL ladder + 880µL H2O + 200µL loading dye = 1200µL ladder mix
dNTP MIX
- made dNTPs according to protocol
- 125µL dATP + 125µL dTTP + 125µL dCTP + 125µL dGTP + 4500µL H2O = 5000µL dNTP Mix @ 10mM (2.5mM each dNTP)
- makes 5 aliquots
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Tara's Lab Notebook
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