User:Tara K. Luckau/Notebook/Team ConGen/2011/09/26

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Contents


sar80

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes



Primer Rehydrations

  • Received next batch of primers from IDT 21Sept2011
  • Rehydrated using LowTE (both F and R):
  • From Lance et al. 2009 (S. undulatus)
Scun7
Scun16
Scun19
  • From Chan et al. 2007 (S. arenicolus)
sar84
SA52.33
SA60.02
  • in prep for a flurry of full gradient PCRs!



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