User:Tara K. Luckau/Notebook/Team ConGen/2011/09/12

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Contents


SCOC MP1 Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • For gel confirmation, used:
    • CPN (row D)
    • 0.1-0.3µL primer amount


  • bands are where expected:
    • Scun10 @ 255-305
    • Scun22 @ 413-453
    • Scun9 @ 257-289
    • Scun15 @ 336-364
  • Gradient in primer volume can kinda be seen in gel, especially in Scun22 primer variation (since all gelled samples amplified)


SCOC MP1 Ship and Fragment Analysis Submission

  • re-hydrated a few wells that had evaporated during thermalcycling
    • Blue: added 7µL to A5 and A6
    • Green: added 3µL to E6
  • FedEx Tracking# 874175289965
  • UAGC Submission#
    • Blue: EHCW
    • Green: KZFP


  • FedEx delivered 9am, 14 September
  • UAGC received 11am, 14 September
  • UAGC completed 3pm, 14 September



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