User:Tara K. Luckau/Notebook/Team ConGen/2011/06/08

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Contents


Scun14

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 160 V
27 µL GelRed 6 µL ladder 1 hour


  • very heavy primer bands under all PCR conditions indicates an inefficient primer set, and will likely cause trace inconsistencies in frag data; not a preferable locus



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