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Weekly Rulon Meeting
Student progress
- Robert has PCRed and geled - got product! But messy. His new goal is to clean it all up - both PCR and gel
- Samantha has also started - rehydrated primer Friday, diluted primer and PCRed Monday
- John started today - rehydrated primer today
- Jess will start primer rehydration this Friday with me
- Sophia and Megan have been told to contact Rulon for Thursday training
Student Primer Assignments
- Samantha = Scun3
- John = Scun6
- Robert = Scun9
- Sophia = Scun10
- Jess = Scun11
- Megan = Scun14
Student Goals
- clean PCR, clean gel
- genotype mass extracted individuals (SCOC SYR9 X04 and SCOC TPIII X06), look for clean peaks, call-ability
- wait for full plates' worth (wait for other students)
- genotype 8-12 individuals from different sites, look for polymorphism
- genotype everyone, enter data
Student Policies
- reiterate that students must complete their lab hours!
- since students will be keeping lab notebook anyway, that will be their time card, too (state date and time of lab work)
- email students to let them know the law of the land:
- weekly meetings are mandatory
- commitment to lab time is mandatory
- failure to meet these minimums will have an impact on grades
What Tara does next
- get Scun2 and Scun22 genotyped
- start with genotyping mass extracted to optimize for pretty
- then panel of 8-12 samples from different sites to check for polymorphism
- so, order some labelled oligos for Scun2 and Scun22
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Tara's Lab Notebook
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