Thoughts and Musings
DNA Concentration
- Tina is prepping mass DNA extraction for optimization (using extra samples with lots of chunky)
- Want to use these samples to figure out relative DNA concentration to use for all future PCR optimizations
- Take Tina's extractions and spot checks
- Run PCR on DNA serial dilution; gel for which concentrations produced product
- note smallest concentration that worked
- shove it through NanoDrop
- Compare that sample's spot check and NanoDrop - that's the brightness of spot we'll want for all future PCRs
Questions
- since I don't really have a verified working primer set, how can I expect the PCR to work?
- will necessarily need to run DNA concentration gradient, temp gradient and buffer gradient in same reaction
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Tara's Lab Notebook
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