User:Tamra L. Fisher/Notebook/Research for Dr. Hartings/2012/01/30

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Miniprep of Transformed NovaBlue E.coli

  • In a sterile test tube with metal cap 10mL of LB with 100╬╝g/mL of ampicillin and one colony from the transformation plate (Friday 1/27/12) were grown overnight (~17 hours). Three of these cultures were prepared.
  • The three cultures were miniprepped using this procedure: Wizard┬« Plus SV Minipreps DNA Purification System
    • The Vacuum Protocol was used
  • The three products of this procedure will be sent off for sequencing at the Johns Hopkins Synthesis & Sequencing Facility



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