User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/18

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Objective

Analyze the intein reactions that were set-up yesterday.

Description

  1. Take fluorescence spectra of the MBP-intein reactions with the gold nanoparticles from yesterday (tubes 7-12).
  2. Also take fluorescence spectra of unreacted (mixed in cuvette, and then immediately placed in the fluorometer) MBP-intein and gold nanoparticle mixtures.

Data

Image:9x_dilute.png

Image:20x_dilute_zoomed.png‎


Image:30x_dilute.png

For the graphs where the "not reacted" data line is not easily seen, it follows the same line as "reacted at 37°C"

Notes

There is a major problem with getting protein gels (SDS-page) to polymerize for use. It would have been helpful to have seen the reaction mixtures on a gel.



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