User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/09/28

From OpenWetWare

Jump to: navigation, search
AU Biomaterials Design Lab Main project page
Previous entry      Next entry


Objective

Biomineralization of gold nanoparticles while keeping the solution at 80°C the entire time. Today we will repeat yesterday's procedure, but use distilled water instead of buffer.

Description

  1. Mix 8mL distilled water, 1mL 15.4μM BSA, and 580μL of 4.3mM Chloroauric acid.
  2. Take 1mL of this solution and transfer it to a quartz cuvette, and place the cuvette in the Shimadzu UV-2550 spectrophotometer at 70°C.
    • Take a spectrum from 200nm-800nm of this every half hour for 3.5 hours.
  3. Put the rest of the solution in the oven set to 80°C for about 3.5 hours.

Data

A spectrum was taken every half hour, and the data collected was corrected with a water spectrum that was taken on 9/7/11:

Because the nanoparticle formation is seen at 550nm, the spectra were zoomed in to better see the increase in absorbance at this wavelength:

The absorbance at 550nm is graphed against time, and it is seen that the absorbance is increasing at 550nm:

Notes

  • The final concentration of BSA is 1.61μM.
    • Calculation:(1mL/9.58mL)*15.4μM
  • The final concentration of Chloroauric acid is 0.26mM.
    • Calculation: (.58mL/9.58mL)*4.3mM



Personal tools