User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2015/02/20

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Feb 20- Bacteria and Strength Objectives Bacterial Inactivation Evaluation in the Dark Agar Plates Saline Solution Synthesize coatings for the metal strength test Analysis of XRD molds Bacterial Inactivation Evaluation in the Dark the tubes containing the bacteria and broth were cloudy, confirming the growth of the bacteria Clean a TiO2/Epoxy film and an Epoxy film with ethanol to kill all bacteria Wash the TiO2/Epoxy film a few times to clean any TiO2 from the surface Clean lab tweezers with ethanol as well. prepare two petri dishes for the films Make sure the film are dried out of the ethanol before placing them in the petri dish Vortex the broth/bacteria tubes Pipette 600 micro liter of the bacteria/broth solution onto the film try to even out the surface as much as you can Cover the petri dish so no evaporation occurs Place in drawer Recipe for Agar Plates Add 3g of tryptone, 1.5 g of yeast, 3 g of NaCl, and 4.5 g of Agar to 300 mL of water Autoclave Saline Solution Recipe 12.5 g of NaCl in 500 mL of water autoclave Strength test The coatings were prepped using half of the amount of weight as normal so that we would have a smaller amount. After mixing a portion was spread onto the bottom piece of metal and covered with the top piece of metal Metal pieces were left to stand until next class to be cured.

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