User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2015/02/20

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Feb 20- Bacteria and Strength

Objectives

  • Bacterial Inactivation Evaluation in the Dark
  • Agar Plates
  • Saline Solution
  • Synthesize coatings for the metal strength test
  • Analysis of XRD molds

Bacterial Inactivation Evaluation in the Dark

  • the tubes containing the bacteria and broth were cloudy, confirming the growth of the bacteria
  • Clean a TiO2/Epoxy film and an Epoxy film with ethanol to kill all bacteria
  • Wash the TiO2/Epoxy film a few times to clean any TiO2 from the surface
  • Clean lab tweezers with ethanol as well.
  • prepare two petri dishes for the films
  • Make sure the film are dried out of the ethanol before placing them in the petri dish
  • Vortex the broth/bacteria tubes
  • Pipette 600 micro liter of the bacteria/broth solution onto the film
  • try to even out the surface as much as you can
  • Cover the petri dish so no evaporation occurs
  • Place in drawer

Recipe for Agar Plates

  • Add 3g of tryptone, 1.5 g of yeast, 3 g of NaCl, and 4.5 g of Agar to 300 mL of water
  • Autoclave

Saline Solution Recipe

  • 12.5 g of NaCl in 500 mL of water
  • autoclave

Strength test

  • The coatings were prepped using half of the amount of weight as normal so that we would have a smaller amount.
  • After mixing a portion was spread onto the bottom piece of metal and covered with the top piece of metal
  • Metal pieces were left to stand until next class to be cured.



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