User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2015/02/13

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Feb 13 -Glassware Objectives To sterilize glassware for antibacterial studies To place films from 02/11/2015 into oven to cure To finish characterizing swelling of epoxy films To prepare a new batch of TiO2/epoxy and epoxy alone films that will be cured in a mold appropriate for XRD testing autoclave broth Measurement of swelling in epoxy film The film made on February 4 was cut into pieces, weighed and measured for thickness, then placed in water until the next lab period Measurements were as follows: Section Initial thickness (mm) Initial weight (g) Final thickness (mm) Final weight (g) % difference in thickness % increase in weights 1 2.17 2.22551 2.30 2.24013 5.99 0.6569 2 1.78 2.23953 1.78 2.25681 0 0.7716 3 1.65 0.93694 1.66 0.94787 0.61 1.1656 4 2.32 2.30817 2.29 2.32841 -1.29 0.8769 Measurement of swelling in epoxy film with average The film made on February 4 was cut into pieces, weighed and measured for thickness, then placed in water until the next lab period Proposed Protocol for the Antimicrobial Activity of the film Grow the bacteria in broth in ambient temperature for two days/or till the solution becomes cloudy Pipette the bacteria/broth solution onto the film in petri dish Use a gauze patch to collect the solution from the film surface Place the gauze patch in an autoclaved NaCl/KCl solution Pipette solution onto a nutrient agar plate and spread over the surface agar plates should be incubated lid down, at 37C for 24 hours before colonies are counted LB Broth Media measure 80 mL of water add 2 g of LB Broth autoclave and sterilize in 121C for 60 minutes, and leave for another hour until the pressure drops back to atmosphere pressure