User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/11/12

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Nov 12 - Beads

Objective

To produce as many beads as possible as a class

Bead Synthesis Protocol

  • 30 mL of ethyl acetate + 500 uL of glutaraldehyde
  • Add your PVA-clay (approximately .5 g of PVA and .051-.055g NaMT) in a dropwise manner. Immediately start timing the reaction for 2 minutes when you do the first drop.
  • After 2 minutes, slowly add 7 mL of 0.2 M NaHCO3.
  • Once completed, transfer to a falcon tube and centrifuge for 1 minute at 3000 rpm, rotor 41.
  • When beads are separated from the organic phase, pipet out the organic phase and re-suspend the beads in distilled water.

The protocol above, was taken from Mary.

Results

The protocol was not able to be massed produced



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