User:Steven Moss/Notebook/AU CHEM-570/2012/03/06

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Spring 2012 Biomaterials Design Lab Main project page
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Objectives

  1. Re-create purple solution without nanoparticles using refolding buffer
  2. Start MBP-intein Protein Expression

Procedure

Studying the Effect of Refolding Buffer (repeat procedure from 12/07/11)

  1. Mix 100μL of 2.76mM HAuCl4 and 100μL 15.06μM BSA with 800μL distilled water in a microcentrifuge tube.
  2. Place the tube on a heat block at 80°C for three hours.
  3. Add 500μL 50mM Tris, pH 7.5.
  4. Remove from heat after about 5 min. Observe.

Begin Expression of MBP-intein

  1. Add 35μL of ampicillin to each of the 250mL flasks (previously autoclaved with 35mL of LB with glucose).
  2. Add one colony from the previously streaked out LB/Amp plate (02/01/12 from glycerol stock pMXB10 Stock #115) to each of these flasks.
  3. Incubate the flasks overnight at 250rpm at 37°C.

Data

  • purple fibers still formed instead of solution

Notes


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