User:Steven J. Koch/Notebook/Kochlab/2010/09/05/Biochem (2003) Hackney et al ADP release, processivity, neck linker spacer

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Contents

Citation

Hackney, D. D., Stock, M. F., Moore, J., & Patterson, R. A. (2003). Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups. Biochemistry, 42(41), 12011-8. http://dx.doi.org/10.1021/bi0349118.

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Notes

  • From the abstract
    • Long neck linker inserts decrease processivity by a factor of 3
    • kinesin with neck linker inserts rapidly release ADP from both heads when binding to a MT, whereas WT kinesin only quickly releases ADP from the head that binds initially
  • They use A25 buffer, which is: 25 mM potassium ACES, pH 6.9,2 mM magnesium acetate, 2m ~ potassium EGTA, 0.1 mM potassium EDTA, and 1 mM 2-mercaptoethanol
    • supplemented with 25 mM KCl, so total ionic strength is 50-60, still lower than BRB80 (PEM80), but higher than Yildiz et al. BRB12

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