User:Steven J. Koch/Notebook/Kochlab/2009/08/28/SapI Oligo Order
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Old notes
Capping hairpin 2
Steve Koch 12:38, 11 August 2009 (EDT): OK, here's a google doc with my notes, and hopefully the same sequence as in the note below: {{#widget:Google Documents |key=0ARLNnjMk2r_qZGdxamtoNnBfMTE0ZDlmeDhjZjM |width=500 |height=300 }}
Capping hairpin
Here is the hairpin I used in grad school:
Unbiotinylated end-cap, HindIII 5’-P-TCGAGGCGACCTAGCGTAAGCTAGGTCGCC Name = P16-Cap-HindIII(incorrect!!!!) L=30 Purification = HPLC Modifications = 5’ phosphate for ligation
I think you should:
- Check with m-fold that it's a hairpin
- replace the 3 or 4 base sticky end with 3 or 4 based for SapI that you have