User:Steven J. Koch/Notebook/Kochlab/2009/04/18/Osmolyte unzipping data 2003/030121 Journal Entries

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These are the journal files from: Popping Paper III\BsoBI Osmolite\030121

Contents

Data set notes

(Copied from http://kochlab.org/files/data/Koch_Data/DataSetNotes/030121.txt)

1/21/03 12:54 PM
Protocol is normal Popping / BGB protocol.  However, Popping buffer is made from DSJ 5x Popping buffer July 2002.  So, THE pH IS NOW 7.5!!!
DNA is end-labeled from December 5 2001.  I diluted 1:2 in BGB 5.
BGB made fresh last week.  Antidig diluted fresh last week. Beads diluted fresh today.
Today will be Betaine studies.  All buffers based on 1x Popping (DSJ), and all enzyme (if present) will be 1:100 dilutions of the BsoBI (same as from Papers)
Sample has more tethers than with 1:5 dilution.  However, the tethers don't seem to be full length (as if nicked)
0149? (Check time)	In 5 mg / ml BGB / popping buffer (DSJ)
0149-0150	Added BsoBI (5 s.v.) (1:50 in 1x DSJ Popping buffer) at about 11:46 AM, after washing with 5 s.v. of 1x DSJ Popping buffer
0151	Flowed in 5 s.v. more of BsoBI at 11:55 AM
0152	Re-trying with new buffer and enzyme.  (Re-diluted 5x DSJ Popping buffer and re-diluted BsoBI in new 1x Pop)  Flowed in 5 s.v. at about 12:10 PM
0153-0154	BsoBI 1:50 in SJK Popping buffer, flowed in 5 s.v. at about 12:19 PM
0155-0157	2/6 M Betain (1:6 dilution of 2M Betain in Popping) plus 1:50 BsoBI @ 12:30 PM

0147.ini



(segment-specific comments below)
(segment000)

0148 Real Time Params.ini

0148.ini

w/ 5 mg / ml BGB

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)

0149 Real Time Params.ini

0149.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)
(segment010)
(segment011)

0150 Real Time Params.ini

0150.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)

0151 Real Time Params.ini

0151.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)

0152 Real Time Params.ini

0152.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)

0153 Real Time Params.ini

0153.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)

0154 Real Time Params.ini

0154.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)

0155 Real Time Params.ini

0155.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)

0156 Real Time Params.ini

0156.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004) Bad FTC?
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)

0157 Real Time Params.ini

0157.ini



(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)

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