User:Steven J. Koch/Notebook/Kochlab/2009/04/18/Osmolyte unzipping data 2003

• /030121 Journal Entries
• /030124 Journal Entries

Steve Koch 15:07, 18 April 2009 (EDT): I found a presentation from 2001 showing that I'd tried out sucrose unzipping. I uploaded this to Google Docs: http://docs.google.com/Presentation?id=dgqjkh6p_46wckm4f3

Preliminary data showed for (among others):

• Unzipping BsoBI in sucrose (osmolite)
• Unzipping cross-linked protein-DNA (UV cross-linking of BsoBI and XhoI)
• Overstretch fluctuations
• Unzipping BsoBI in high calcium (shows increased unzipping force, and increased non-specific binding)

Steve Koch 16:37, 18 April 2009 (EDT): Also found a whole slew of old presentations. Lots of cool unpublished results in these things. I should start uploading all of them, but I don't want to get too distracted now. In any case, here is a cool one where I show that EcoRI DFS had a cool bimodal distribution: http://docs.google.com/Presentation?id=dgqjkh6p_13g62rp7cf

2003 Presentation summarizing betaine DFS

• http://docs.google.com/Presentation?id=dgqjkh6p_72hfv46jnw
• Media:030129 Meeting MDW 1 M Betaine DFS.ppt